Immortalized human podocyte cell line

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WitrynaMouse Podocyte Cell Line Mpc 5, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more ... WitrynaG.J. Audesirk, in Comprehensive Toxicology, 2010 13.24.2.3.5 Immortal cell lines. Immortal cell lines are an alternative to primary cultures. Frequently used cell lines …

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WitrynaATCC cell lines immortalized human podocytes ab8 13 m saleem Cell Lines Immortalized Human Podocytes Ab8 13 M Saleem, supplied by ATCC, used in … WitrynaCell Lines. HK2 cells (ATCC) were cultured in Dulbecco’s Modified Eagle Medium Low Glucose (DMEM LG) supplemented with 10% foetal bovine serum (FBS) (Euroclone S.p.A., Pero, MI, Italy). Immortalized human podocytes, previously characterized were cultured in DMEM High Glucose (Euroclone) with 10% FBS. Glomerular endothelial … did netherlands win the world cup

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WitrynaSteroid-resistant nephrotic syndrome (SRNS) is a frequent cause of CKD. The discovery of monogenic causes of SRNS has revealed specific pathogenetic pathways, but these monogenic causes do not explain all cases of SRNS. To identify novel monogenic WitrynaThese results suggest that podocyte cell lines can be established consistently from human urine. The generation of podocyte cell lines from urine of patients and … WitrynaPlating of glomeruli onto a collagen surface is followed by an outgrowth of cobblestone-like cells that, after replating, differentiate into arborized, mature podocytes. Currently, the majority of research studies use immortalized podocytic cell lines most often derived from transgenic mice bearing a conditional immortalizing gene. did netlfix have a student discount

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WitrynaThe protein products of these canonical podocyte genes are essential for the proper podocyte function. In cell culture, human and mouse podocytes also lose expression of NPHS1 and NPHS2, 23, 24 ... WitrynaA stable SPL-kd cell line of human podocytes was generated by the lentiviral shRNA transduction method and was characterized for reduced SPL mRNA and protein … did netherlands won world cupWitryna1 kwi 2002 · The conditionally immortalized human podocyte cell line AB8/13 was kindly provided by M. Saleem and has been described previously [8, 40]. Podocytes were cultured in the medium which contained ... did neuman\\u0027s bakery go out of business

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Immortalized human podocyte cell line

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Witryna14 lut 2015 · Cell culture and high glucose-induced podocytopathy: An immortalized mouse podocyte cell line 25 which had been generated by expression of a temperature-sensitive mutant of the SV40 Large-T ... WitrynaLoss of glomerular podocytes is the crucial event in the progression of chronic kidney disease (CKD). tRNA-derived fragments (tRFs), a newfangled bran…

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Witrynaeases. A conditionally immortalized human podocyte cell line has been developed by transfection with the temperature-sen-sitive SV40-Tgene. These cells … WitrynaConditionally immortalized human podocyte WT and CRISPR/CAS9 GLA KO cell lines were differentiated at 37 °C at 70% confluency for 10 to 14 days. Growth protocol Conditionally immortalized human podocytes were let to proliferate at 33°C in RPMI-1640 medium supplemented with 10% fetal calf serum (FCS), Penicillin/Streptomycin, …

WitrynaCell line name: CIHP-1: Synonyms: Conditionally Immortalized Human Podocytes-1; AB8/13; AB 8/13; hiPOD; ciPOD: Accession: CVCL_W186: Resource Identification … WitrynaA conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression. J Am Soc Nephrol. 2002; 13: 630-638. Crossref; PubMed; Google Scholar, 20. Saleem M.A. Ni L. Witherden I. et al. Co-localization of nephrin, podocin, and the actin cytoskeleton: evidence for a role in podocyte foot process formation.

WitrynaA breakthrough in cell biology research. Human telomerase reverse transcriptase (hTERT)–immortalized primary cells represent a breakthrough in cell biology … WitrynaThe conditionally immortalized human podocyte cell line AB8/13 was kindly provided by M. Saleem and has been described previously [9,74]. Podocytes were cultured in the medium which contained RPMI 1640 medium with 10% fetal bovine serum, and insulin–transferrin–selenium at 33 °C. For the differentiation, podocytes were …

Witryna14 lut 2015 · Mouse podocytes immortalised by transduction with a temperature sensitive large T antigen SV40 construct as previously described 45, 46, were used …

WitrynaATCC cell lines immortalized human podocytes ab8 13 m saleem Cell Lines Immortalized Human Podocytes Ab8 13 M Saleem, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more did newark have an nfl or pre-nfl franchiseWitrynaThe human podocytes derived from the stem cells discussed in this review have shown some advantages over the immortalized podocytes, but their maturation remains a question that has not been compared with that of the human adults in-vivo. 42,43,89 Among the various reports on the induction of podocytes and kidney organoids from … did netherland won the world cupWitryna25 mar 2024 · conditionally immortalized cell line. 1 reference. described by source. A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression. 1 reference. A novel source of cultured podocytes. 1 reference. derived from organism type. Homo sapiens. did neverland ranch sellWitryna11 lis 2009 · Conditionally immortalized human podocytes were cultured for up to 24 h with 375–750 μM palmitate. Functional effects on glucose uptake and ceramide production were measured. ... Reiser J, et al. A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression. J Am Soc … did neville end up with lunaWitrynaHuman cell lines are a critical biological resource. These in vitro models of human biology are used in biomedical research, toxicology studies, bioindustry, drug … did new brunswick want confederationWitryna3 sty 2024 · In this study, conditionally immortalized human podocytes were used, ... This podocyte cell line proliferates under permissive conditions at 33 °C. When cultivated at 37 °C, the SV40 T-antigen is inactivated for cell differentiation. The culture medium for human podocytes was RPMI 1640 Medium (Gibco, via Thermo Fisher … did new amsterdam go off the airWitryna18 kwi 2024 · The immortalized human podocyte cell line were purchased from Bristol University and cultured under their published protocol. Podocyte mRNA profiles of these cells were examined. Contributor(s) Yoshimura Y, Nishinakamura R: Citation(s) 30635375: Submission date: Jun 30, 2024: Last update date: Apr 18, 2024: did new amsterdam become new york