Phosphomotif antibodies

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August undefined, 2024

WebPrimary Antibodies (9010) Secondary Antibodies (76) Antibody Conjugates (1994) Activators & Inhibitors (224) Buffers & Dyes (108) Cellular Assay Kits (118) ChIP Kits & Reagents (156) CUT&RUN Kits & Reagents (15) Cytokines & Growth Factors (215) ELISA Kits (513) Experimental Controls (248) Flow Cytometry Kits & Reagents (9) Proteomic Analysis … WebJan 4, 2013 · These antibodies are commonly used in immunoprecipitation and/or immunoblotting protocols to determine kinase-induced substrate phosphorylation. This …

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Webnonphosphorylatable Ser172Ala mutation abolished interaction with the antibody to the 14-3-3 binding motif, whereas a nonphosphorylatable Ser155Ala mutation abolished the interaction with the antibody to the MPK substrate motif, and mutation of either site alone did not affect the AICAR-induced phosphorylation of the other site (Fig. 3C). WebFeb 2, 2024 · Antibody buffer and 0.5 μg of antibody were added to samples and incubated overnight at 4°C. After washing beads, 2.5 μl of pAG-NMase was added and incubated for 10 min at RT. After washing, 1 μl of chromatin digest additive was added and incubated for 2 hours at 4°C. Stop buffer and 0.5 ng of Spike-in DNA were then added and incubated for ... inchkeith house mental health edinburgh

Using phospho-motif antibodies to determine kinase substrates

WebNov 26, 2013 · Here we have used phosphomotif antibodies and mass spectrometry (MS) to investigate the effects of MEK/PI3K dual inhibition during the period immediately preceding cell death. WebJan 9, 2014 · After 2D-DIGE analysis, changed protein spots were characterized for reactivity to ERK phosphomotif antibodies and identified by MS. Various types of phosphomotif … Webever, phosphomotif antibodies have not been used so far for large scale proteomics identification of kinase substrates. To discover novel MAPK targets at the synapse, we raised a phosphospecific antibody against a peptide library repre-senting the MAPK consensus phosphorylation motif. Using this antibody we affinity-purified putative MAPK ... inchkeith house cmht

Phosphomotif antibody 5557 is phosphospecific and …

Phosphosite-Specific Antibodies: A Brief Update on …

WebThe validation of phosphosite-specific antibodies requires multiple techniques and tactics to demonstrate their specificity. These antibodies can be used in arrays, flow cytometry, and imaging platforms. The specificity of phosphosite-specific antibodies is vital for their use in proteomics and profiling of disease. less Based on techniques WebPhosphomotif Antibody Generation and Purification Phosphorylated and non-phosphorylated peptide libraries were chemically synthesized with C-terminal cysteine or … inazuma farming route inazuma f2p weapons

"WebApr 12, 2024 · This antibody only detected protein when amino acids 166-575 were present, suggesting the antibody epitope resides within this region. The faint bands found in all lanes are likely the endogenous WAC protein isoforms expressed by the HEK293T cells. Overall, these data demonstrated that we could express the various WAC mutant proteins and ... " - Phosphomotif antibodies

Phosphomotif antibodies

Identification of Phosphorylation Consensus Sequences and …

WebThis study describes a method for the identification of the substrates of specific serine kinases. An antibody specific for the phosphomotif generated by the kinase is used to … WebProtocols. As the epigenetics experts, Active Motif continues to develop new methods and helpful techniques to support your research. Our goal is to ensure your success with everything from gold standard assays to emerging approaches for epigenetics analysis. Check back often as we add new protocols, technotes and guides to this resource.

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WebProteins were extracted from each cell line and digested with trypsin. For phosphopeptide enrichment in serial immunoaffinity purifications, phosphomotif antibodies were used in the following order: PXpSP, pTP, RXRXXpS, and RXXpS where “X” represents any amino acid and “p” reflects the phosphorylation site. The samples were run in ... WebApr 1, 2009 · Phosphomotif antibody 5557 is phosphospecific and detects MAPK phosphorylation events.A, sequence of phosphopeptide library containing two …

WebOct 13, 2010 · Both studies 2,3 used phosphomotif-specific antibodies for immunoaffinity purification before MS analysis and quantified the effects of various pathway inhibitors on the newly identified Ser/Thr ... WebApr 11, 2024 · Ph2AX antibody was initially tested on cisplatin-treated and untreated iKras PDAC cells embedded in agarose, and an optimal dilution of 1:400 was determined for the detection of DNA damage cells by IHC staining. Primary and secondary OmniMap HRP-conjugated antibodies were used in 100 μl volumes, for 60 and 16 min, respectively.

WebFeb 11, 2013 · The scaffolding adapter protein Gab2 (Grb2-associated binder) participates in the signaling response evoked by various growth factors and cytokines. Gab2 is … WebPhosphospecific antibodies developed against key drug-regulated phosphorylation sites provide an ideal tool for this approach because they can be used for …

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WebJan 15, 2016 · To examine endogenous MFF phosphorylation, we developed a phosphospecific antibody to phospho-Ser 172 (P-Ser 172), which we validated for … inchkeith house clinicWebPhosphoproteomic screen for PDK4 substrates, followed by nonphosphorylatable and phosphomimetic mutations of the PDK4 site revealed cytoplasmic GTPase, Septin 2 (SEPT2), as the key effector … inazuma fight musicWebATM-substrate phosphomotif blots showed a single, low molecular weight feature which similarly decreased in cells when PI3K was inhibited (i.e., in cells treated with PI3Ki alone … inchkeith islandWebApr 13, 2024 · Figure 1.Expression levels of obscurin in wild-type and Obscn-ΔIg58/59 atria.(A,A’) Immunoblotting (A) and relative quantification (A’) of giant obscurin in wild-type 12-month-old left ventricles (LV) and atrial lysates utilizing antibodies to Ig67 did not reveal significant expression differences; t-test, p = 0.18.(B) Immunoblotting of giant obscurin in … inchkeith island ferryWebPhosphoSitePlus Protein or Substrate Search Alternative Search Options Protein, Sequence, or Reference Search Protein, Sequence, or Reference Search: Protein Searches retrieve lists of proteins and their modification types based on protein name or ID, protein type, domain, cellular component, MW, and pI range. inazuma fishing locations mapWebThe validation of phosphosite-specific antibodies requires multiple techniques and tactics to demonstrate their specificity. These antibodies can be used in arrays, flow cytometry, and … inchkeith house podiatryWebDec 8, 2015 · Together, using a TNIK inhibitor and phosphomotif antibody, we identify endogenous substrates of TNIK in neurons, define consensus sequences for TNIK, and suggest signaling pathways by which... inazuma fishing rod