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Tailed duplex

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Single-stranded DNA versus tailed duplex in sequence …

Web1 Jul 2001 · Surprisingly, a 3′-tailed duplex, a standard substrate for 3′→5′ helicases, is unwound much less efficiently by BLM and WRN than are the bubble and X-structure … WebResearch has shown that TteUvrD is able to displace blunt-ended and 5‘-ssDNA tailed duplex. However, researchers determined, that event if TteUvrD prefers 3‘ssDNA tailed duplexes, its unwinding activity is much lower than seen in E. coli UvrD helicase [4] . stark county jr 4h https://charlotteosteo.com

SV40 T-antigen uses a DNA shearing mechanism to initiate origin ...

Web10 Feb 2010 · (F) ATPase domain of archaeal Hel308 bound to a 3′-tailed duplex (Buttner et al., 2007). The ATPase domain of either the UvrD (E) or Hel308 interacts extensively with … Web16 Nov 2024 · Background Single-stranded (ss) DNAs are utilized in various molecular biological and biotechnological applications including the construction of double-stranded … WebThe EMBO Journal Vol.19 No.5 pp.1148–1156, 2000 Tailed duplex DNA is the preferred substrate for Rad51 protein-mediated homologous pairing Alexander V.Mazin, Elena … stark county job \u0026 family services canton oh

Reconstitution of the strand invasion step of double-strand break ...

Category:Improved Gene Correction Efficiency with a Tailed Duplex …

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Tailed duplex

The formation of adjacent triplex–duplex domains in DNA

Web25 Nov 2009 · The biological relevance of this robust and “unconventional” 5′-tailed duplex strand invasion has so far not been fully explored, most probably because of the … http://microbiology.ucdavis.edu/kowalczykowski/PDF_files/CHEMTRACTS,%202400,%2013,%20415-423.pdf

Tailed duplex

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Web1 Dec 2024 · Various diseases, including cancer, are caused by genetic mutations. A 5′-tailed duplex (TD) DNA, consisting of a long single-stranded (ss) editor DNA and a short … Web5 Sep 2024 · A model summarizing one way in which 5′ ssDNA tailed duplexes can arise in an area of the genome (Ter sites) that is targeted for new spacer acquisition during …

WebTailed Duplex DNA Is the Preferred Substrate for Rad51 Protein-Mediated Homologous Pairing Research by A.V. Mazin, E. Zaitseva, P. Sung, and S.C. Kowalczykowski, EMBO J. … http://microbiology.ucdavis.edu/kowalczykowski/PDF_files/Mazin%20et%20al.%20(2000)%20EMBO%20J%2024,%201148-1156.pdf

Web26 Oct 2024 · Duplex sequencing involves tagging both strands of DNA with random, but complementary, double-stranded primers. The molecules are then amplified, sequenced, and grouped by their tags. Errors that are introduced from PCR or sequencing can be filtered out by looking for discrepancies among molecules with the same tag. Web23 Sep 2016 · A tailed-duplex DNA substrate was then designed by annealing T21 and C20 oligonucleotides for the analysis of this study (Materials and Methods). The resulting DNA …

Web12 Aug 2005 · When the 3′-ssDNA tailed duplex was used, unwound single-stranded product increased as a function of increasing enzyme concentration ( Fig. 3A ). In parallel unwinding assays, Tte-UvrD displayed a significant unwinding activity toward blunt-ended ( Fig. 3B) and 5′-ssDNA tailed duplex ( Fig. 3C ).

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